RESUMO
The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.
Assuntos
Apresentação de Antígeno/imunologia , Compostos de Cálcio , Células Dendríticas/imunologia , Antígenos de Histocompatibilidade Classe I/imunologia , Nanopartículas , Silicatos , Células Apresentadoras de Antígenos/imunologia , Antígenos Virais/química , Antígenos Virais/imunologia , Compostos de Cálcio/química , Células Cultivadas , Células Dendríticas/metabolismo , Epitopos/administração & dosagem , Epitopos/imunologia , Humanos , Nanopartículas/química , Nanopartículas/toxicidade , Nanopartículas/ultraestrutura , Tamanho da Partícula , Fragmentos de Peptídeos/administração & dosagem , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/imunologia , Silicatos/química , Propriedades de SuperfícieRESUMO
The paper aims at studying optical properties of porous silicon powders and thin films which were impregnated with different porphyrin molecules. It has been shown that introducing porphyrins into porous silicon matrix results in quenching of luminescence from porous silicon, while luminescence of porphyrins survives, though its structure changes. At the same time, porphyrins in porous silicon matrix which was preliminarily oxidized does not alter luminescence from porphyrins. Generation of singlet oxygen by illuminated porphyrin/porous silicon composite is confirmed by additional oxidation of porous silicon and by the observation of characteristic 1270 nm luminescence band.
Assuntos
Materiais Revestidos Biocompatíveis/química , Medições Luminescentes , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Porfirinas/química , Silício/química , Adsorção , Materiais Revestidos Biocompatíveis/efeitos da radiação , Luz , Teste de Materiais , Nanoestruturas/efeitos da radiação , Ligação ProteicaRESUMO
A model of articular cavity with smooth cover was produced by influence of shape-forming element on regenerating bone tissue. Morphological, ultrastructural, histochemical, and biochemical peculiarities of the tissue of the formed articular surface are described.
Assuntos
Cartilagem Articular/ultraestrutura , Articulação do Quadril/citologia , Ílio/citologia , Cápsula Articular/anatomia & histologia , Acetilglucosamina/análise , Animais , Cartilagem Articular/química , Cartilagem Articular/citologia , Sulfatos de Condroitina/análise , Citoplasma/diagnóstico por imagem , Cães , Fibroblastos/diagnóstico por imagem , Ácido Hialurônico/análise , Hidroxiprolina/análise , Microscopia Eletrônica , Modelos Teóricos , Proteoglicanas/análise , Valores de Referência , Propriedades de Superfície , Membrana Sinovial/química , Membrana Sinovial/diagnóstico por imagem , UltrassonografiaRESUMO
Diagnostic possibilities of selective examination of renal urine particularly collected under medicamentous polyuria conditioned by the administration of Lazix were studied in latent bacteriuria. By means of separate collection of renal urine against the background of polyuria it was possible additionally to detect bacteriuria in 1/3 of the patients, to record increase in the intensity of the index in almost half of the patients with renal bacteriuria and, in 1/4 of them to detect, in renal urine, the aetiological agent absent from bladder urine. The latter circumstance not only has a diagnostic significance but also plays a certain role in the selection of medicamentous therapy in chronic pyelonephritis. In addition to traditional bacteriological methods, filtration through membrane filters was used to isolate and identify microflora in the urine. By means of this method it is possible to detect extremely low bacteriuria which cannot be established by any other method.
